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. Author manuscript; available in PMC: 2020 Jun 15.
Published in final edited form as: Cancer Res. 2019 Sep 24;79(24):6084–6100. doi: 10.1158/0008-5472.CAN-19-2132

Figure 7.

Figure 7.

DNp63 alters ErbB family signaling profile in the ‘neural’ LK2 cells. A, Human phospho-receptor tyrosine kinase array was performed for GFP-overexpressed and DNp63-overexpressed LK2 cells. B, Protein expression of ErbB4, phospho-ErbB4, ErbB3, phospho-ErbB3, EGFR, phospho-EGFR, phospho-Akt and phospho-Erk1/2 and β-Actin as a loading control in GFP-overexpressed and DNp63-overexpressed LK2 cells. Cells were serum-starved for 4 hours, and then incubated with anti-ErbB4 monoclonal antibody at the indicated concentrations for 15 minutes, followed by stimulation with NRG1 for 30 minutes at the indicated concentrations. C, Cell proliferation of GFP-overexpressed and DNp63-overexpressed LK2 cells. Cells were cultured with 5% FBS and with or without NRG-1 (100 ng/ml) and anti-ErbB4 monoclonal antibody (25 μg/ml) for 4 days. Mean ± SD of sextuplicates are shown. **, P<0.001 and N.S., not significant (P>0.05), t-test with Bonferroni correction.