KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| trans-Palmitoleic acid | Cayman Chemical, MI | Cat#9001798; CAS:10030-73-6 |
| Methyl Palmitate | Sigma-Aldrich, MO | Cat#P5177; CAS: 112-39-0 |
| 11-cis Vaccenyl Acetate | Cayman Chemical, MI | Cat#10010101; CAS: 6186-98-7 |
| Methoprene | Sigma-Aldrich, MO | Cat#33375; CAS: 40596-69-8 |
| Ethanol | Sigma-Aldrich, MO | Cat#E7023; CAS: 64-17-5 |
| Focusclear | CelExplorer Labs Co., Taiwan | Cat#FC-101 |
| Critical Commercial Assays | ||
| RNeasy Mini Kit | QIAGEN, Germany | Cat#74104 |
| QIAshredder Kit | QIAGEN, Germany | Cat#79654 |
| SuperScript VILO Master Mix | Thermo Fisher Scientific, MA | Cat#11755050 |
| iQ SYBR Green Supermix | Bio-Rad Laboratories, CA | Cat#1708880 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: or47b2 | [53]; Bloomington Drosophila Stock Center | BDSC: 51306 |
| D. melanogaster: Or47b-GAL4 | [54]; Bloomington Drosophila Stock Center | BDSC: 9984 |
| D. melanogaster: Or47b-GAL4 | [54]; Bloomington Drosophila Stock Center | BDSC: 9983 |
| D. melanogaster: LexAop2-6XGFP | [55]; Bloomington Drosophila Stock Center | BDSC: 52265 |
| D. melanogaster: UAS-CaMKI-RNAi | [56]; Bloomington Drosophila Stock Center | BDSC: 26726 |
| D. melanogaster: UAS-dCBP-RNAi | [57]; Bloomington Drosophila Stock Center | BDSC: 32576 |
| D. melanogaster: ey-FLP | Bloomington Drosophila Stock Center | BDSC: 5580 |
| D. melanogaster: Tub-(FRT-GAL80-FRT)-STOP | [58]; Bloomington Drosophila Stock Center | BDSC: 38880 |
| D. melanogaster: UAS-traF | [59]; Bloomington Drosophila Stock Center | BDSC: 4590 |
| D. melanogaster: fruF | [60]; Bloomington Drosophila Stock Center | BDSC: 66873 |
| D. melanogaster: U6-fru-sgRNA | [36]; Bloomington Drosophila Stock Center | BDSC: 80225 |
| D. melanogaster: CaMKI[EY07197] | [62]; Bloomington Drosophila Stock Center | BDSC: 16799 |
| D. melanogaster: UAS-dCas9-VPR | [36]; Bloomington Drosophila Stock Center | BDSC: 66561 |
| D. melanogaster: UAS-CaMKII-RNAi | [56]; Bloomington Drosophila Stock Center | BDSC: 29401 |
| D. melanogaster: fru[GAL4.P1] | [21]; Bloomington Drosophila Stock Center | BDSC: 66696 |
| D. melanogaster: UAS-Met-RNAi | Vienna Drosophila Stock Center | VDRC: 100638 |
| D. melanogaster: UAS-fru-miRNA | [61] | N/A |
| D. melanogaster: fru[UAS.P1] | This study | N/A |
| D. melanogaster: 10XUAS-mLexA-VP16-NFATΔC | This study | N/A |
| Oligonucleotides | ||
| fruP1 forward primer: GCCACGCCCACTCGCATTAC | This study | N/A |
| fruP1 reverse primer: TGGTCAGTGTTGTACCTAG | This study | N/A |
| rp49 forward primer: AGGGTATCGACAACAGAGTG | This study | N/A |
| rp49 reverse primer: CACCAGGAACTTCTTGAATC | This study | N/A |
| fruP1 gRNA: CACATAAACGCAGTACATGG TGG | This study | N/A |
| Recombinant DNA | ||
| Plasmid: pJRC81 | [63] | Addgene, Cat#104615 |
| Plasmid: 10XUAS-mLexA-VP16-NFATΔC | This study | N/A |
| Software and Algorithms | ||
| MATLAB | MathWorks | RRID:SCR_001622; https://www.mathworks.com/products/matlab.html |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| pCLAMP 10.7 | Molecular Devices | RRID: SCR_011323; http://www.moleculardevices.com/products/software/pclamp.html |
| Igor Pro V.6 | Wavemetrics | RRID:SCR_000325; https://www.wavemetrics.com/products/igorpro/ |
| GPower 3.1 | [67]; Universität Düsseldorf | http://www.gpower.hhu.de |
| CFX Manager | Bio-Rad Laboratories, CA | Cat# 1845000 |
| Other | ||
| Aluminosilicate glass electrodes | Sutter Instrument Co., CA | AF100-64-10 |
| Vacuum desiccator | Cole-Parmer, IL | VX-06514-30 |
| Fluorescent dust | LDP LLC, NJ | UVXPBR |