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. Author manuscript; available in PMC: 2020 May 30.
Published in final edited form as: Cell. 2019 Apr 25;177(6):1507–1521.e16. doi: 10.1016/j.cell.2019.03.045

Figure 3-. Steady state levels of Fe-S containing proteins are restored when FXN null cells are grown in hypoxia.

Figure 3-

(A) Aconitase activity assay from control or FXN KO K562 cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592. Bar plots show mean ± SD. ****=p < 0.0001. One-way ANOVA with Bonferroni’s post-test. (B) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted for OXPHOS subunits or Tubulin. (C) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted for Lipoic acid, which is conjugated to PDH-E2, KGDH-E2 and GCSH. Additional blots against OGDH (i.e. KGDH-E1) and Actin. (D) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted against enzymes in the heme biosynthesis pathway- FECH and ALAD- or TOMM20. (E) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted against POLD1, MMS19 or Actin. See also Figure S3.