(A) The C. elegans frataxin null mutant, frh-1(tm5913), carries a 353 bp deletion and must be propagated as a balanced heterozygote at room air. (B) When incubated at 1% O2
frh-1(tm5913) mutants develop to adulthood and are fertile. Pictured are wild type animals grown for 2 days and frh-1(tm5913) mutants grown for 4 days. Scale bar = 3 mm. (C) Total progeny produced from animals incubated at 21% O2 or 1% O2. Mothers were balanced heterozygotes (mutant/+). (D) Immunoblot for lipoic acid in animals grown at 1% O2 or animals shifted as adults to normoxia for the indicated time. (E) hsp-6::gfp fluorescence in animals grown in 1% O2 or shifted to normoxia as adults for 4 days. Exposure time = 50 ms, scale bar = 500 μm. (F) Animal length after 4 days growth at 21% or 50% O2. Mothers were balanced heterozygotes frh-1(tm5913)/+. (G) Animal length after 3 days growth in hyperoxia. Mothers were balanced heterozygotes frh-1(tm5913)/+. All error bars represent standard deviation. *=p < 0.05, **=p < 0.01, ***=p < 0.001, ****=p < 0.0001. One-way ANOVA with Bonferroni’s post-test. See also Figure S6.