Figure 3.

RNA-seq identifies DEGs in PD-L1–altered relative to PD-L1–unaltered DLBCL. Heatmap (A) and volcano plot (B) depicting 137 DEGs in DLBCLs with vs without PD-L1 gene alterations (false discovery rate-adjusted P < .05, fold change ≥1.5 or ≤−1.5). (C) GO analysis performed on the 137 DEGs. GO terms significantly enriched in DLBCLs with PD-L1 gene alterations are denoted by red dots. (D) IPA revealing predicted upstream regulators of gene expression that are activated (positive z score) and inhibited (negative z score) in DLBCLs with PD-L1 alterations relative to those without. Dashed vertical line indicates the position of P = .05 on the x-axis. (E) GSEA demonstrating enrichment of NF-κB–regulated genes in PD-L1–altered compared with PD-L1–unaltered DLBCLs. IFN-γ, interferon-γ; NES, normalized enrichment score; PTEN, phosphatase and tensin homolog.