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. Author manuscript; available in PMC: 2020 Jan 12.

Published in final edited form as: Eur Respir J. 2019 Dec 12;54(6):1900378. doi: 10.1183/13993003.00378-2019

Figure 4: — Sprague Dawley (SD) male rats weighing 210–245 grams (Charles River Laboratories) were housed in chambers under normoxia or hypoxic (high altitude ~ 18,000 feet) conditions for four weeks. Rats were dosed with vehicle methylcellulose (0.5%)/Tween-80 (0.5%) or PT2567 (300 mg/kg/day), beginning the day they were placed in chambers. After 4 weeks, endpoint measurements for the experimental animals were conducted (A–C). A) Mean Pulmonary Arterial Pressure. B) Ratio of weight of right ventricle versus weight of left ventricle. C) Representative images of pulmonary vessels stained with anti-macrophages/monocytes antibody, clone ED-1 (Top), anti-Ki67 antibody (Middle), or anti-Tenascin C antibody (Bottom). The levels of CD68, CCNA1, and TNC mRNAs in the whole lung tissues of indicated rats were also shown (Right). D, E) α-SM-actin positive pulmonary vessels, scale bar: 100μm. Statistical significance determined by 2-way ANOVA (A, B) or by t-test (C–E).

Figure 4: — Sprague Dawley (SD) male rats weighing 210–245 grams (Charles River Laboratories) were housed in chambers under normoxia or hypoxic (high altitude ~ 18,000 feet) conditions for four weeks. Rats were dosed with vehicle methylcellulose (0.5%)/Tween-80 (0.5%) or PT2567 (300 mg/kg/day), beginning the day they were placed in chambers. After 4 weeks, endpoint measurements for the experimental animals were conducted (A–C). A) Mean Pulmonary Arterial Pressure. B) Ratio of weight of right ventricle versus weight of left ventricle. C) Representative images of pulmonary vessels stained with anti-macrophages/monocytes antibody, clone ED-1 (Top), anti-Ki67 antibody (Middle), or anti-Tenascin C antibody (Bottom). The levels of CD68, CCNA1, and TNC mRNAs in the whole lung tissues of indicated rats were also shown (Right). D, E) α-SM-actin positive pulmonary vessels, scale bar: 100μm. Statistical significance determined by 2-way ANOVA (A, B) or by t-test (C–E).