Table 1.
Plasmids and strains used in this study.
| Plasmids | Description | Source |
|---|---|---|
| pET21c | T7 promoter, AmpR | Novagen |
| pBR322 | AmpR, TetR | Thermo Scientific |
| pET28a | T7 promoter, KanR | Novagen |
| pCDFDuet-1 | double T7 promoters, StrpR | Novagen |
| pACYCDuet-1 | double T7 promoters, CmR | Novagen |
| pTS | pET21c carrying tryptophan regulatory element (tnaC operon) and tetA gene, AmpR | This study |
| pTC | pET21c carrying the tetA gene, AmpR | This study |
| pTE0 | pET28a carrying the trpEfbr gene under the control of the T7 promoter, KanR | Wang et al. (2019) |
| pTE3-1 | pUC57(KanR) carrying the trpEfbr, pctV and shiA genes under the control of the constitutive Zymomonas mobilis pyruvate decarboxylase promoter (Ppdc), KanR | Wang et al. (2019) |
| pTE3 | pET28a carrying the trpEfbr and aroGfbr genes under the control of the constitutive Zymomonas mobilis pyruvate decarboxylase promoter (Ppdc), KanR | Wang et al. (2019) |
| pTY0 | pET28a carrying the trpEfbr and TDC genes under the control of the T7 promoter, KanR | This study |
| pTY1 | pET28a carrying the trpEfbr, aroGfbr and TDC genes under the control of the T7 promoter, KanR | This study |
| pTY2 | pET28a carrying the trpEfbr, aroGfbr and TDC genes under the control of the constitutive Zymomonas mobilis pyruvate decarboxylase promoter (Ppdc), KanR | This study |
| pTD2-1 | pUC57(KanR) carrying the trpD and trpC genes under the control of the constitutive Zymomonas mobilis pyruvate decarboxylase promoter (Ppdc) and trpB and trpA genes under the control of the T7 promoter, KanR | Wang et al. (2019) |
| pTY3 | pET21c carrying the trpD and trpC genes under the control of the constitutive Zymomonas mobilis pyruvate decarboxylase promoter (Ppdc), AmpR | This study |
| pTY4 | pET28a carrying the TDC gene under the control of the T7 promoter, KanR | This study |
| pTY5 | pET21c carrying the TDC gene under the control of the constitutive Zymomonas mobilis pyruvate decarboxylase promoter (Ppdc), AmpR | This study |
| pTD2 | pACYCDuet-1 carrying the trpD, trpC, trpB and trpA genes under the control of the constitutive Zymomonas mobilis pyruvate decarboxylase promoter (Ppdc), CmR | Wang et al. (2019) |
| pSE1 | pET21c carrying the hipA gene under the control of the mtr promoter (Pmtr), AmpR | Wang et al. (2019) |
| pTD3 |
pCDFDuet-1 carrying the trpD, trpC, trpB and trpA genes under the control of the constitutive Zymomonas mobilis pyruvate decarboxylase promoter (Ppdc), StrpR |
This study |
| Strains |
Description |
Source |
| BL21(DE3) | F− ompT hsdSB (rB–, mB–) gal dcm (DE3), a common expression host | Invitrogen |
| BH2 | E. coli BL21(DE3) ΔxylA ΔtyrA ΔpheA, an engineered strain with disrupted xylose catabolism, as well as tyrosine and phenylalanine pathways | Zhang et al. (2015a) |
| XL10-Gold | endA1 glnV44 recA1 thi-1 gyrA96 relA1 lac Hte Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 TetRF’[proAB lacIqZΔM15 Tn10(TetR) Amy CmR], a strain with high transformation efficiency | Stratagene |
| BTH3 | BH2 harboring pTE3 and pTD2 | Wang et al. (2019) |
| BTP1 | BTH3 harboring pET21c | Wang et al. (2019) |
| XYD | XL10-Gold harboring pET28a and pTY5 | This study |
| BMC | BH2 harboring pTY2 and pTD2 | This study |
| BMC2 | BH2 harboring pTY2, pTD2 and pTC | This study |
| BLXC | BL21(DE3) harboring pTC | This study |
| BLXS | BL21(DE3) harboring pTS | This study |
| XTH | XL10-Gold harboring pTE3 and pTD3 | This study |
| BTXC | BTH3 harboring pTC | This study |
| BTS | BTH3 harboring pTS | This study |
| BMS | BH2 harboring pTY2, pTD2 and pTS | This study |
| BTS1 | BTH3 harboring pSE1 | Wang et al. (2019) |
| BMS1 | BH2 harboring pTY2, pTD2 and pSE1 | This study |