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. 2019 Oct 28;38(24):e102566. doi: 10.15252/embj.2019102566

Figure EV1. Effect of SGO2 knockdown on expression of inducible genes by other stimuli.

Figure EV1

MEF cells were infected for 72 h with adenovirus expressing shRNA for SGO2 (SGO2‐KD1 or SGO2‐KD2), HSF1, p300, CBP, or scrambled RNA (SCR). Amphiregulin (AREG) and CXCL1 mRNA levels during treatment with forskolin (20 μM) for 1 h were quantified by RT–qPCR, and the levels relative to those in control SCR‐treated cells are shown (left upper). The forskolin‐mediated induction was reduced by p300 or CBP knockdown (Takii et al, 2015), but not by SGO2 or HSF1 knockdown. The mRNA levels of LHX15 and RAP2B, HSF1‐independent doxorubicin‐inducible gene products (Fujimoto et al, 2018), during the treatment with doxorubicin (0.5 μM) for 24 h were also quantified by RT–qPCR (left lower). Cell extracts were prepared using NP40‐lysis buffer and subjected to immunoblotting (right). Error bars, SD (n = 3). Asterisks indicate *P < 0.05 or **P < 0.01 by Student's t‐test. An arrowhead indicates SGO2 band, and an asterisk indicates non‐specific band in SGO2 blot.