Figure 8.

MYST1 regulates EGF expression possibly via H4K16 acetylation. A, The correlations of MYST1 mRNA level with the levels of several EGFR ligands including EGF, TGFA, AREG, and EREG in the datasheet from the GlioVis. B and C, ELISA assay was performed to detect the EGF protein levels in the medium of LN229 and U87 cells after MYST1 silencing or overexpression and cultured for 48 h within 10 mL medium. D, Relative mRNA levels of EGF in LN229 and U87 cells after MYST1 silencing or overexpression. E and F, Western blot was performed to detect the protein levels of H4K16 acetylation in LN229 and U87 cells after MYST1 silencing or overexpression. Histone H4 and GAPDH was used as controls. G, ChIP‐seq data of H4K16ac in human fibroblast IMR90 cells using chip antibodies H4K16ac (Millipore 07‐329, GSM1358821_H4K16ac.Prolif.R1) or H4K16ac (Abcam ab109463, GSM1358822_H4K16ac.Prolif.R2) was downloaded from GEO and analyzed by using the IGV 2.6.3 software. All data were used as mean ± SD, n ≥ 3, significant difference was tested by Student's t test. *P < .05, **P < .01, ***P < .001. P < .05 were considered as statistically significant. EGFR, epidermal growth factor receptor; ELISA, Enzyme‐linked immuno sorbent assay