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. 2019 Nov 18;38(24):e101822. doi: 10.15252/embj.2019101822

Figure EV1. Fo5176 hyphae and elicitors cause an immediate speed decrease of plasma membrane localized cellulose synthase complexes.

Figure EV1

  1. GFP‐CesA3 speed at the plasma membrane of WT (Col‐0) root cells 5 min after half MS treatment or Fo5176 contact (as depicted in Fig 1A). Box plots: centerlines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend to the minimum and maximum. N ≥ 369 particles from 6 cells and 6 roots and three independent experiments; Welch's unpaired t‐test; ***P‐value ≤ 0.001.
  2. GFP‐CesA3 speed at the plasma membrane of WT (Col‐0) root cells 5 min after half MS or half MS + elicitor treatment (as depicted in Fig 1F). Box plots as described in (A). N ≥ 647 particles from 9 cells and 6 roots and three independent experiments; Welch's unpaired t‐test; ***P‐value ≤ 0.001.
  3. GFP‐CesA3 speed at the plasma membrane of WT (Col‐0) root cells 5 min after being exposed to half MS + 5 mM MES and half MS + 5 mM MES + elicitor treatment (as depicted in Fig 2A). Box plots as described in (A). N ≥ 786 particles from 13 cells and 11 roots and three independent experiments.
  4. Growth rate of WT (Col‐0) roots exposed to fungal elicitors and 5 mM MES. After 17 min of growth in half MS + 5 mM MES, H2O or elicitors were added and the growth rate was measured for an additional 17 min. Average growth rate before treatment (−17 to 0 min): 5 mM MES: 0.0074 ± 0.0009 μm/s; elicitors + 5 mM MES: 0.0068 ± 0.0010 μm/s. Average growth rate after treatment (0–17 min): 5 mM MES: 0.0077 ± 0.0024 μm/s; elicitors + 5 mM MES: 0.0069 ± 0.0020 μm/s. Values are mean ± SEM, N ≥ 11 seedlings from three independent experiments. Welch's unpaired t‐test for roots before and after elicitor treatment; P‐value = 0.33.

Source data are available online for this figure.