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. 2019 Nov 18;38(24):e101822. doi: 10.15252/embj.2019101822

Figure 3. Fo5176 hyphae do not affect the cellulose synthase machinery and growth rate of cc1cc2 mutant roots.

Figure 3

  1. Representative image of a 5‐day‐old WT (Col‐0) YFP‐CesA6 and mCh‐TUA5 dual‐labeled root epidermal cell under mock conditions (left panel) or upon 5 min of Fo5176 hyphae contact (right panel). A green dashed line in the bright field (BF) channel highlights Fo5176 hyphae. Scale bar = 5 μm.
  2. Representative image of a 5‐day‐old cc1cc2 YFP‐CesA6 and mCh‐TUA5 dual‐labeled root epidermal cell under mock conditions (left panel) or upon 5 min of Fo5176 hyphae contact (right panel). A green dashed line in the BF channel highlights Fo5176 hyphae. Scale bar = 5 μm.
  3. YFP‐CesA6 density at the plasma membrane of WT (Col‐0) or cc1cc2 root cells after Fo5176 hyphae contact as depicted in (A and B). Box plots: centerlines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend to the minimum and maximum. N ≥ 21 cells from 10 roots and three independent experiments; Welch's unpaired t‐test; *P‐value ≤ 0.05, ***P‐value ≤ 0.001.
  4. Microtubule density at the cell cortex of WT (Col‐0) or cc1cc2 root cells after Fo5176 hyphae contact as depicted in (A and B). Box plots as described in (C). N ≥ 24 cells from 9 roots and three independent experiments; Welch's unpaired t‐test; ***P‐value ≤ 0.001.
  5. Growth rate of cc1cc2 roots grown in half MS or half MS + Fo5176 hyphae. Average growth rate in half MS: 0.0046 ± 0.0005 μm/s; average growth rate in half MS + Fo5176 hyphae: 0.0045 ± 0.0003 μm/s. Values are mean ± SEM, N = 17 seedlings from three independent experiments. Welch's unpaired t‐test; P‐value = 0.78.
  6. Representative image of a 5‐day‐old cc1cc2 GFP‐CC1 and mCh‐TUA5 dual‐labeled root epidermal cell under mock conditions (left panel) or upon 5 min of Fo5176 hyphae contact (right panel). A green dashed line in the BF channel highlights Fo5176 hypha. Scale bar = 5 μm.
  7. Representative image of a 5‐day‐old cc1cc2 GFP‐CC1ΔN120 and mCh‐TUA5 dual‐labeled root epidermal cell under mock conditions (left panel) or upon 5 min of Fo5176 hyphae contact (right panel). A green dashed line in the BF channel highlights Fo5176 hypha. Scale bar = 5 μm.
  8. GFP‐CC1 or GFP‐CC1ΔN120 density (as proxy for the CSC) at the plasma membrane of cc1cc2 root cells after Fo5176 hyphae contact as depicted in (F and G). Box plots as described in (C). N ≥ 22 cells from 8 roots and three independent experiments; Welch's unpaired t‐test; *P‐value ≤ 0.05, **P‐value ≤ 0.01.
  9. Microtubule density at the cell cortex of cc1cc2 GFP‐CC1 or cc1cc2 GFP‐CC1ΔN120 root cells after Fo5176 hyphae contact as depicted in (F and G). Box plots as described in (C). N ≥ 24 cells from 7 roots and three independent experiments; Welch's unpaired t‐test; **P‐value ≤ 0.01, ***P‐value ≤ 0.001, n.s.  = not significant.

Source data are available online for this figure.