FIG 2.

Biochemical variation (measured by ¹H NMR spectroscopy) between the gut contents of donor horses and the in vitro gut model supernatant from the concordance study. (A) PCA scores plot for PC1 and PC2 of the model (R² = 0.64). (B and C) The loading plot for PC1, describing 50% of the total variance associated with the higher concentration of acetate in model supernatant at time points T1 to T5 (B), and the loading plot for PC2, describing 14% of the total variance associated with the higher acetate concentration in gut contents (C). PC, principal component; T, turnover.