FIG 1.

Transcriptional analysis of genes affected by HHP treatment in B. subtilis. B. subtilis strain TI465 was subjected to HHP treatment at 250 MPa at 25°C for 10 min. After HHP treatment, the culture was diluted 5-fold in fresh NaCl-free L medium and incubated at 37°C with vigorous shaking. After incubation for 0 h (immediately after dilution), 2 h, or 4 h, cells were collected by centrifugation. Then total RNAs were extracted and used for RT-qPCR analysis. The relative expression was expressed as the relative amount of each transcript per 1,000 units of 16S rRNA (ΔCt). Genes are classified into four groups according to their functions: translation-related genes (A), stress-responsive genes (B), metabolic genes (C), and metal-regulated genes (D). The average values of at least four independent experiments are shown.