TABLE 2.
Mutations identified following whole-genome sequencing in L. reuteri-derivatives used in this study
| Gene ID (JCM1112) | Annotation | DNA sequence change (amino acid sequence change)a
|
|||
|---|---|---|---|---|---|
| ΔФ1 | ΔФ2 | SH | ΔФ1 ΔФ2 | ||
| LAR_0044 | FmtB (Mrp) protein | - | - | - | 1268–1270delATA; 3-bp deletion |
| LAR_0099 | ParB, chromosome partitioning | - | - | 268G→T (A90S) | 268G→T (A90S) |
| LAR_0011 | DNA-binding response regulator | - | - | - | 371C→T (T124I) |
| LAR_1262 | GentR family transcription regulator | 298G→A (V100I) | - | 298G→A (V100I) | 298G→A (V100I) |
| LAR_1266 | IS30 family transposon | 257G→A (S86N) | - | 257G→A (S86N) | 257G→A (S86N) |
| LAR_1268 | Dextransucrase protein | - | - | - | 107_108insC; frameshift mutation |
a
-, identical to the wild-type sequence; ΔΦ1, L. reuteri 6475 mutant in which prophage 1 is deleted; ΔΦ2, L. reuteri 6475 mutant in which prophage 2 is deleted; SH, sensitive host lacking both prophages and their attB sites; ΔΦ1 ΔΦ2, derivative of SH in which attB sites were restored.