FIG 7.
Complementation of m153 abrogates Clr-b loss observed in m153-deficient virus. NIH 3T3 cells were transduced with pTRIPZ lentivirus expressing m153 or empty vector and infected with MCMV. (A) Comparison of the Clr-b expression on resting transduced cells. (B) Histograms of Clr-b levels on NIH 3T3.vector and NIH 3T3.m153HA fibroblasts upon infection with MCMV (WT, Δ6, or Δm153). (C and D) Analysis of NIH 3T3.vector cells by quantitation of Clr-b MFI (C) and quantitation of percent Clr-b– cells at different MOI (D). (E and F) Analysis of NIH 3T3.m153HA cells by quantitation of Clr-b MFI (E) and quantitation of percent Clr-b– cells at different MOI (F). Data were analyzed using 2-way ANOVA and are representative of those from 3 independent experiments.