FIG 8.
Clr-b is stabilized by m153 through an indirect mechanism. (A and B) Clec2d transcript levels in NIH 3T3 cells infected with WT or Δm153 virus (A) and in NIH 3T3 cells transduced with m153HA or empty control pTRIPZ vector (B). (C and D) Immunoprecipitations using anti-FLAG or anti-HA MAb from lysates of NIH 3T3.Clr-bFLAG cells transfected with m153HA or empty control vector. These lysates were immunoblotted for anti-FLAG (C) or anti-HA (D). (E) NIH 3T3 cells were transfected with constructs of m153 and Clr-b fused to either EGFP or mCherry and analyzed for cellular localization using fluorescence microscopy 48 h posttransfection. All data are representative of those from at least 3 independent experiments.