TABLE 1.
Primers used in this study
| Gene | Primer | Sequence (5′–3′) |
|---|---|---|
| Cloning of m02 family and m145 familya | ||
| N-terminally HA tagging m153 | ||
| m153HA | m153 NT-HA Fwd1 | TTGCTTACCCATACGATGTTCCAGATTACGCTGGATCAGGATCAGAGGTCGTGCGGCCCGAAGT |
| m153 NT-HA Xho F2 | gcctcgagATGTCTGCACTTCTGATCCTAGCTCTTGTTGGAGCTGCAGTTGCTTACCCATACGATGTT | |
| m153 NotI R | ggcggccgcTCACACCACATTCTCCTCCGTA | |
| Construction of type I m153 reporters | ||
| m153 EC | m153 XhoI ATG Fwd | gctcgagATGATTCCCCTTCTCCTTCTGCCG |
| m153 NF-PSP Fwd | gcctcgagATGTCTGCACTTCTGATCCTAGCT | |
| m153 EC Rev | tagcggccgcGGTCAGTCTCGAATCGTTGATCGTCCTCTGG | |
| Construction of Tet-inducible pTRIPZ vectors | ||
| m153 | m153 NT-HA AgeI-ATG F | ataaccggtcgccaccATGTCTGCACTTCTGATCCTAGC |
| m153 XhoI-TGA R | tatctcgagTCACACCACATTCTCCTCCGTATCCG | |
| Clr-b | Clr-b AgeI-ATG F | ataaccggtcgccaccATGTGTGTCACAAAGGCTTCC |
| Clr-b C-FLAG XhoI-TGA R | tatctcgagCTACTTGTCGTCGTCGTCCTTGTAGTCTGATCCTGATCCGGAAGGAAAAAAAGGAGTTTGG | |
| Construction of EGFP/mCherry fusion constructs | ||
| m153 | m153 fusion XhoI F | ggactcagatctcgagcgccaccATGTCTGCACTTCTGATCCTAGCTCTTGTTGGAGC |
| m153 fusion BamHI R | ggcgaccggtggatccCCTGATCCTGATCCCACCACATTCTCCTCCGTATCCGAGCACTCG | |
| Clr-b | Clr-b fusion XhoI F | atctcgagGATCAGGATCAATGTGTGTCACAAAGGCTTCC |
| Clr-b fusion BamHI R | gtggatccTCACTTGTCGTCGTCGTCCTTGTAGTCTGATCCTGATCCGGAAGGAAAAAAAGGAGTTTGGCAGTGG | |
| qRT-PCR primers | ||
| m153 | m153 qPCR Fwd | GTGTCAGATGACGACCCAGG |
| m153 qPCR Rev | TCTGACTTCTGTTGACCGGC |
a
The complete m02 and m145 family were cloned as previously described (36).