FIG 5.

NS2 palmitoylation plays a role in NS2 and E2 localization to the DRM. Shown are the results of a membrane flotation assay performed following cold 1% Triton X-100 treatment of cell lysates harvested from cells electroporated with wt or NS2/C113S(TCA) mutated 2E3 RNA at 72 h postelectroporation (see Materials and Methods for details). The relative percentage of each protein in the DRM fractions was calculated by dividing the levels of protein detectable from the top 4 fractions by those from all fractions. The graph at the bottom shows the mean percentage, with standard deviation, of each protein detected in the DRM fractions from three independent experiments.