FIG 3.
NSP4 coimmunoprecipitates with the COPII proteins Sec23 and Sec24, but not Sec31. Immunoprecipitations were performed with mock (M)- and rotavirus (RV)-infected cell lysates using antibodies against Sec23, Sec24, and Sec31 and analyzed by Western blotting. (Upper panel) A Western blot was probed for NSP4 using rabbit peptide-specific antibody αNSP4114–135. (Lower panel) NSP4, Sec23, Sec 24, Sec31, and GAPDH were detected in the input lysates using the indicated protein-specific antibodies.