FIG 9.

ER transmembrane marker proteins do not colocalize with NSP4 surrounding viroplasms. Rotavirus-infected cells were fixed at 6 hpi (A and B) or 4 hpi (C) and stained to detect calnexin (green) (A) or SERCA (green) (B and C), NSP4 (red), viroplasms (NSP5, purple) and nuclei (blue). In panel C, an asterisk (*) denotes infected cells in which SERCA and NSP4 colocalize. Rightmost panel C shows a magnified view of the boxed area in panel C (Merge), which shows early colocalization of SERCA with NSP4 that is subsequently lost by 6 hpi shown in panel B. Scale bars: 5 μm (A and B) and 10 μm (C).