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APH-2 and HRS interact in EEA1-positive but not CD63-positive endosomes. HeLa cells were transfected with 750 ng of GFP-APH-2 (green) and 250 ng of control plasmid (A and C) or HA-HRS (B) expression plasmid; 24 h posttransfection, endogenous EEA1 or CD63 was detected using antibodies against EEA1 (A and B) or CD63 (C) epitopes, followed by Alexa Fluor 594-conjugated secondary antibody (red). Nuclei were stained with DAPI (blue). Scale bars, 10 μm.
