Skip to main content
. 2019 Dec 16;51(6):730–744.e6. doi: 10.1016/j.devcel.2019.10.016
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Mouse Anti-NeuN IgG1 Clone A60, 1:500 Millipore Cat#MAB377, RRID:AB_2298772
Mouse Anti-Caspr IgM, 1:50 Dr. Matthew Rasband N/A
Mouse Anti-NF200, 1:20000 Sigma Cat#N0142, RRID:AB_477257
Mouse Anti- Kv 1.1 IgG2b, 1:200 Neuromab Cat#75–105, RRID:AB_2128566
Rabbit Anti-MBP Pep-7, 1:1000 Dr. Peter Brophy N/A
Rabbit Anti-MNF2 (Anti-Nfasc186), 1:1000 Dr. Peter Brophy N/A
Goat Anti-Mouse AlexaFlour 488, 1:1000 Thermofisher Cat#A-21141, RRID:AB_2535778
Goat Anti-Mouse AlexaFlour 594, 1:1000 Thermofisher Cat#A-21125, RRID:AB_2535767
Goat Anti-Mouse AlexaFlour 647, 1:1000 Thermofisher Cat#A-21240, RRID:AB_2535809
Goat Anti-Mouse FITC, 1:100 Southern Biotec Cat# 1021-02, RRID:AB_2794237
Goat Anti-Rabbit AlexaFlour 488, 1:1000 Molecular Probes Cat# A-11008, RRID:AB_143165
Donkey Anti-Rabbit AlexaFlour 594, 1:1000 Jackson ImmunoResearch Cat#111-585-14,RRID:AB_2307325

Experimental Models: Organisms/Strains

Zebrafish: Tg(mbp:EGFP-CAAX) (Almeida et al., 2011) ZFIN: ZDB-ALT-120103-2
Zebrafish: Tg(mbp:nlsEGFP) (Karttunen et al., 2017)
Zebrafish: Tg(NBT:DsRed, Synon. Tg(Xla.Tubb:DsRed), zf148Tg (Peri and Nüsslein-Volhard, 2008) ZDB-ALT-081027-2
Tg(cntn1b:mCherry) (Czopka et al., 2013) ZDB-TGCONSTRUCT-140610-5
Zebrafish: Tg(sox10:KalTA4) (Almeida and Lyons, 2015) N/A
Tg(cldnk:Gal4) (Münzel et al., 2012) ZDB-TGCONSTRUCT-120207-1
Zebrafish: Tg(UAS:Mem-GFP) (Kwan et al., 2007) N/A
Zebrafish: casprsa12772 (Kettleborough et al., 2013) ZDB-ALT-130530-311
Zebrafish: nfascbue56 this paper N/A
Mouse: Nfasc-/-/Nfasc186 (Zonta et al., 2011) N/A
Mouse: Caspr-/- (Gollan et al., 2003) N/A

Oligonucleotides

Morpholino: ATG-nfascb,
TGACAGGAATCCTCCAACACTTCAT
Gene Tools N/A
Morpholino: Human Beta-Globin, Standard Negative Control,
CCTCTTACCTCAGTTACAATTTATA
Gene Tools N/A
nfascb_ORF_F ACAAAATTACACCAAAACGCTGT N/A
nfascb_ORF_R AGCCAGTCTGACAGATTAAATGGA N/A
nfascb_geno143_F TTGCATGCCTGAGCAGAATA N/A
nfascb_geno143_R TACTTTCCTCCTCGGCTCCT N/A
Caspr_geno803_F TTTCACACCAACAACATGGAA N/A
Caspr_geno803_R GGGAAGGGTGGATGGAATAA N/A
Nfascb_179-975_RT_F TCCAGTCTTCACATGGACGC N/A
Nfascb_179-975_RT_R CGGAACCGATTTGACCCTGA N/A
mbpX3_RT_F AGAAAGGGAAAGAGACCCCAC N/A
mbpX3_RT_R GATCGGCTTTCTCCCAGGTT N/A
nfasc_FACS-RT_F GGCCCTCCTAAACCAGACAC N/A
nfasc_FACS-RT_R ATGGGTTTGAAGCGTTGCAC N/A
rbfox3a_FACS-RT_F AGGGACCAGCAGCTTAACAC N/A
rbfox3a_FACS-RT_R GCGACTGTAACCTCCTCTGT N/A
myrf_FACS-RT_F AATCGTTCTGGGGAACTCGG N/A
myrf_FACS-RT_R GATCGTTAGCTTGCTGGGGT N/A
Caspr_FACS-RT_ F TTTCCGGAAGAACCGTCTGG N/A
Caspr_FACS-RT_ R CTTCCCCCTTGTAGCCTGTG N/A
attB1_nfascb_F GGGGACAAGTTTGTACAAAAA
AGCAGGCTGCCACCATGAAGTG
TTGGAGGATTCCTGTC
N/A
Nfascb_noStop18_F CTCGCCCTTGCTCACCATAGCAAA
AGAGTAGATGGCCACAGGAGATGT
GGGCTCAGAG
N/A
18_EGFP_F GCCATCTACTCTTTTGCTATGGTGA
GCAAGGGCGAGGAGCTGTTCACC
GGGGTGG
N/A
attB2R_EGFP_R GGGGACCACTTTGTACAAGAAAGCTG
GGTTTACTTGTACAGCTCGTCCATGCC
N/A
attB4_nfascb_F GGGGACAACTTTGTATAGAAAAGTTGAA
AAAACCTCCCACACCTCCCCC
N/A
attB1R_nfascb_R GGGGACTGCTTTTTTGTACAAACTTGGC
CACCATGAAGTGTTGGAGGATTCCTGTC
N/A

Recombinant DNA

pTol2- UAS:nfascb-EGFP-pA This paper N/A
pTol2- nfascB-EGFP-UAS-mCherry This paper N/A
Tol2 Kit (Kwan et al., 2007) http://tol2kit.genetics.utah.edu/index.php/ Main_Page

Software and Algorithms

Zen, Zen Blue Zeiss RRID:SCR_013672
Fiji (Schindelin et al., 2012) RRID:SCR_002285
GraphPad Prism GraphPad software RRID:SCR_015807
Adobe Photoshop Adobe RRID:SCR_014199