REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse Anti-NeuN IgG1 Clone A60, 1:500	Millipore	Cat#MAB377, RRID:AB_2298772
Mouse Anti-Caspr IgM, 1:50	Dr. Matthew Rasband	N/A
Mouse Anti-NF200, 1:20000	Sigma	Cat#N0142, RRID:AB_477257
Mouse Anti- Kv 1.1 IgG2b, 1:200	Neuromab	Cat#75–105, RRID:AB_2128566
Rabbit Anti-MBP Pep-7, 1:1000	Dr. Peter Brophy	N/A
Rabbit Anti-MNF2 (Anti-Nfasc186), 1:1000	Dr. Peter Brophy	N/A
Goat Anti-Mouse AlexaFlour 488, 1:1000	Thermofisher	Cat#A-21141, RRID:AB_2535778
Goat Anti-Mouse AlexaFlour 594, 1:1000	Thermofisher	Cat#A-21125, RRID:AB_2535767
Goat Anti-Mouse AlexaFlour 647, 1:1000	Thermofisher	Cat#A-21240, RRID:AB_2535809
Goat Anti-Mouse FITC, 1:100	Southern Biotec	Cat# 1021-02, RRID:AB_2794237
Goat Anti-Rabbit AlexaFlour 488, 1:1000	Molecular Probes	Cat# A-11008, RRID:AB_143165
Donkey Anti-Rabbit AlexaFlour 594, 1:1000	Jackson ImmunoResearch	Cat#111-585-14,RRID:AB_2307325
Experimental Models: Organisms/Strains
Zebrafish: Tg(mbp:EGFP-CAAX)	(Almeida et al., 2011)	ZFIN: ZDB-ALT-120103-2
Zebrafish: Tg(mbp:nlsEGFP)	(Karttunen et al., 2017)
Zebrafish: Tg(NBT:DsRed, Synon. Tg(Xla.Tubb:DsRed), zf148Tg	(Peri and Nüsslein-Volhard, 2008)	ZDB-ALT-081027-2
Tg(cntn1b:mCherry)	(Czopka et al., 2013)	ZDB-TGCONSTRUCT-140610-5
Zebrafish: Tg(sox10:KalTA4)	(Almeida and Lyons, 2015)	N/A
Tg(cldnk:Gal4)	(Münzel et al., 2012)	ZDB-TGCONSTRUCT-120207-1
Zebrafish: Tg(UAS:Mem-GFP)	(Kwan et al., 2007)	N/A
Zebrafish: casprsa12772	(Kettleborough et al., 2013)	ZDB-ALT-130530-311
Zebrafish: nfascbue56	this paper	N/A
Mouse: Nfasc-/-/Nfasc186	(Zonta et al., 2011)	N/A
Mouse: Caspr-/-	(Gollan et al., 2003)	N/A
Oligonucleotides
Morpholino: ATG-nfascb, TGACAGGAATCCTCCAACACTTCAT	Gene Tools	N/A
Morpholino: Human Beta-Globin, Standard Negative Control, CCTCTTACCTCAGTTACAATTTATA	Gene Tools	N/A
nfascb_ORF_F	ACAAAATTACACCAAAACGCTGT	N/A
nfascb_ORF_R	AGCCAGTCTGACAGATTAAATGGA	N/A
nfascb_geno143_F	TTGCATGCCTGAGCAGAATA	N/A
nfascb_geno143_R	TACTTTCCTCCTCGGCTCCT	N/A
Caspr_geno803_F	TTTCACACCAACAACATGGAA	N/A
Caspr_geno803_R	GGGAAGGGTGGATGGAATAA	N/A
Nfascb_179-975_RT_F	TCCAGTCTTCACATGGACGC	N/A
Nfascb_179-975_RT_R	CGGAACCGATTTGACCCTGA	N/A
mbpX3_RT_F	AGAAAGGGAAAGAGACCCCAC	N/A
mbpX3_RT_R	GATCGGCTTTCTCCCAGGTT	N/A
nfasc_FACS-RT_F	GGCCCTCCTAAACCAGACAC	N/A
nfasc_FACS-RT_R	ATGGGTTTGAAGCGTTGCAC	N/A
rbfox3a_FACS-RT_F	AGGGACCAGCAGCTTAACAC	N/A
rbfox3a_FACS-RT_R	GCGACTGTAACCTCCTCTGT	N/A
myrf_FACS-RT_F	AATCGTTCTGGGGAACTCGG	N/A
myrf_FACS-RT_R	GATCGTTAGCTTGCTGGGGT	N/A
Caspr_FACS-RT_ F	TTTCCGGAAGAACCGTCTGG	N/A
Caspr_FACS-RT_ R	CTTCCCCCTTGTAGCCTGTG	N/A
attB1_nfascb_F	GGGGACAAGTTTGTACAAAAA AGCAGGCTGCCACCATGAAGTG TTGGAGGATTCCTGTC	N/A
Nfascb_noStop18_F	CTCGCCCTTGCTCACCATAGCAAA AGAGTAGATGGCCACAGGAGATGT GGGCTCAGAG	N/A
18_EGFP_F	GCCATCTACTCTTTTGCTATGGTGA GCAAGGGCGAGGAGCTGTTCACC GGGGTGG	N/A
attB2R_EGFP_R	GGGGACCACTTTGTACAAGAAAGCTG GGTTTACTTGTACAGCTCGTCCATGCC	N/A
attB4_nfascb_F	GGGGACAACTTTGTATAGAAAAGTTGAA AAAACCTCCCACACCTCCCCC	N/A
attB1R_nfascb_R	GGGGACTGCTTTTTTGTACAAACTTGGC CACCATGAAGTGTTGGAGGATTCCTGTC	N/A
Recombinant DNA
pTol2- UAS:nfascb-EGFP-pA	This paper	N/A
pTol2- nfascB-EGFP-UAS-mCherry	This paper	N/A
Tol2 Kit	(Kwan et al., 2007)	http://tol2kit.genetics.utah.edu/index.php/ Main_Page
Software and Algorithms
Zen, Zen Blue	Zeiss	RRID:SCR_013672
Fiji	(Schindelin et al., 2012)	RRID:SCR_002285
GraphPad Prism	GraphPad software	RRID:SCR_015807
Adobe Photoshop	Adobe	RRID:SCR_014199