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. 2019 Nov 1;8(11):1374. doi: 10.3390/cells8111374

Figure 7.

Figure 7

(A) Senescence-associated beta-galactosidase staining was increased in SW1353 cells treated with IL1β (b) compared to controls (a); vitamin B6′s supplementation counteracted IL1β-induced senescence (c). (B) The generation of ROS induced by IL1β in SW1353 was reduced by adding vitamin B6 in cells medium. (C) Similarly, vitamin B6′s supplementation (c) was able to reduce IL1β-induced apoptosis (b) in SW1353 cells. (D) The increased COX2 gene expression and the reduced expression of chondrogenic genes due to IL1β treatment were reverted thanks to vitamin B6 supplementation. (E) Vitamin B6 ability in counteracting IL1β effects in SW1353 was confirmed by Alcian blue staining evaluating GAG production: (a) Controls; (b) IL1β treated; (c) IL1β + vit B6 treated; magnifications: A: 5X (Well plate) and 10X (culture slides); C: 20X; E: 5X; * p ≤ 0.05; *** p ≤ 0.0005; **** p ≤ 0.000005. (* CTRL vs. IL1β, * IL1β vs. IL1β+ Vit B6).