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Effects of piperlongumine (PL) on cell cycle-regulatory proteins in MCF-7 cells. The expression of cyclin B1, p-CDK1, CDK1, PCNA, cyclin D1, CDK6, and CDK4 at mRNA (A) and protein levels (B) was measured using real time PCR and Western blotting, respectively. GAPDH was used as an internal control gene and as a loading control. Data represent the mean ± SEM (n = 3). * Significantly different compared with the control (p < 0.05).
