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. 2019 Nov 14;8(11):553. doi: 10.3390/antiox8110553

Figure 6.

Figure 6

Effects of piperlongumine (PL) on p21 mRNA expression in MCF-7 cells. The mRNA level of p21 in PL-treated cells was measured using real time PCR. (A) Cells were treated with PL (0, 10, and 20 µM) for 12 h. (B) Cells were treated with Bay 11-7082 (Bay; NF-κB inhibitor; 10 and 20 µM) or PL (10 and 20 µM) for 12 h, with or without NAC pretreatment (5 mM, 1 h). GAPDH was used as the internal control gene. Data represent the mean ± SEM (n = 3). * Significantly different compared with the control (p < 0.05). # Significantly different compared to 20 µM of PL (p < 0.05).