Differentially expressed proteins in si-hVDAC1-TTs and si-NT-TTs subjected to short- and long-term treatment. (A) Human proteins differentially expressed between tumors treated with si-NT or si-hVDAC1 for short or long periods, as analyzed by LC-HR-MS/MS. Out of 1,845 human proteins presenting at least 1 unique peptide, those with a p-value of 0.05 and linear fold change of +/− 1.5 are presented. (B) Volcano plots showing p-values as a function of fold change in si-hVDAC1-TTs treated short (B) or long term (C), relative to si-NT-TTs. Cutoff lines shown are as in (A), namely a p-value of 0.05 and a linear fold change of +/−1.5. (D) Hierarchical clustering of the 167 proteins found to be differentially expressed after either short- or long-term treatment. The color scale of the standardized expression values is shown. (E,F) Significantly enriched functional groups based on GO biological process (E) and GO cellular component (F) in the long-term si-hVDAC1-TTs. Shown are GO terms with a Fisher’s exact test FDR-adjusted p-value < 0.05 and more than 4 differentially expressed proteins. The X-axis shows the –log10 of the p-value.