Figure 2.

IHC analysis of B-cell lymphoma 2 (Bcl2) expression with H-score in CTCcl− vs. CTCcl+ triple-negative breast cancer (TNBC) PDX tumors. Primary tumor tissues were sectioned and stained individually with an anti-Bcl2 antibody after antigen retrieval. Sections were exposed with metal enhanced 3,3′-Diaminobenzidine (DAB) substrate and counter-stained with hematoxylin. Histoscore was assigned based on staining intensity (0 (absent), 1+ (weak), 2+ (moderate), or 3+ (strong)) and the percentage of cells stained.