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. 2019 Nov 1;8(11):1831. doi: 10.3390/jcm8111831

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of mitotic capacity after coating material administration. (A–F) Immunofluorescence analysis of phospho-Histone 3 (Ser 10) proteins in non-treated (A) or coating material-challenged (B–E) cells. The concentrations of materials were indicated on the panel. Cells were incubated with specimens for 24 h. Nuclear 4′,6-diamidino-2-phenylindole staining is shown in blue. (F) The quantification of phospho-Histone 3 (Ser 10)-positive cell proportions was analyzed. Nuclear 4’,6-diamidino-2-phenylindole (DAPI) staining is shown in blue. The scale bar is 50 µm.