Table 2.
Determination of optimal concentration of exosomal proteins for cryopreservation of dog sperm after incubation 4 °C for 8–10 h.
| Groups | Motility (%) | Linearity (%) | Straightness (%) | ALH (µm) | Live sperm (%) |
|---|---|---|---|---|---|
| Control | 75.1 ± 0.6 b | 26.4 ± 1.0 | 47.1 ± 0.7 | 4.4 ± 0.3 | 67.9 ± 0.3 a |
| 25 µg/ mL | 73.8 ± 0.3 b | 24.5 ± 0.6 | 45.9 ± 0.7 | 4.7 ± 0.1 | 64.8 ± 3.5 b |
| 50 µg/ mL | 77.8 ± 0.2 a | 26.3 ± 0.3 | 47.5 ± 1.1 | 5.0 ± 0.1 | 68.6 ± 0.1 a |
| 100 µg/ mL | 73.9 ± 0.5 b | 26.6 ± 0.9 | 46.2 ± 1.6 | 4.7 ± 0.1 | 64.9 ± 0.3 b |
ALH, amplitude of lateral head displacement. Values with different superscripts letters in a column differ significantly (p < 0.05, n = 4).