Figure 5.

Pseudouridylation activity of D. discoideum dyskerin mutants. RNA was purified from wild-type (AX4) or dyskerin mutant (knockin-1, knockin-2) strains and treated, or not, with N-cyclohexil-N′-(2-morpholinoethyl) carbodiimide metho-p-toluenesulphonate (CMCT). The amount of unmodified sno18 (yellow bars) or 26S rRNA (blue bars) was determined by RT-qPCR. The relative amount of RNA determined for treated versus untreated samples was referred to that obtained for AX4 RNA. Mean values and standard deviation obtained in triplicate experiments are represented.