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. 2019 Nov 8;8(11):1406. doi: 10.3390/cells8111406

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of mRNAs coding for snoRNP component in D. discoideum dyskerin mutants. RNA was isolated from Wild-type (AX4) and dyskerin mutant (knockin 1, knockin 2) strains. The expression of the mRNAs coding for the snoRNP components dyskerin (dkc), GAR1 (gar1), NHP2 (nhp2) and NOP10 (nop10) was determined by RT-qPCR. Expression data were normalized using the large mitochondrial rRNA as control. Expression values were related to those of the AX4 strain for each gene. Mean values and standard deviations from three independent experiments are represented. * p < 0.05; *** p < 0.001.