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. 2019 Nov 8;8(11):1406. doi: 10.3390/cells8111406

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A1 — Dkc1 expression in clones obtained by homologous recombination. RNA was isolated from different clones that had incorporated dkc1 by homologous recombination, as determined by the PCR test shown in Figure 2. The relative expression of dkc1 mRNA was determined by reverse transcription and quantitative PCR and compared to that of AX4 cells. Panel A shows the results obtained for several clones corresponding to the knockin-1 generation. Clone numbers are shown in the X-axes. The arrow indicates the clone that was selected for further analysis. Panel B shows the relative expression of the knockin-1 and knockin-2 clones selected for the study after removal of the Blasticidin resistance cassette upon expression of the Cre recombinase. This result is in agreement with the data on dkc1 expression shown in Figure 6.