Figure 4.

Response to pro-inflammatory cytokine (TNFα); (A) median fluorescence intensity (MFI) of ICAM-1 in hiPSC-ECs cultured in basic conditions (complete medium)—mean ± SEM, data is taken from at least 3 independent differentiation experiments; (B) representative dot plot; (C) increase in ICAM-1 expression presented as percentage from respective control cells (cells not treated with the cytokine), showing no difference in ICAM-1 increase between the normal hiPSC-ECs and cells with introduced mutation in HNF1A gene (sgHNF1A shows summarized data from both clones); (D) increase in ICAM-1 expression presented as percentage from respective control cells (cells not treated with the cytokine), showing no difference in ICAM-1 increase between the normal hiPSC-ECs (control 2) and cells with monoallelic or biallelic mutation in HNF1A gene.