Figure 5.

Permeability assay of hiPSC-ECs; (A) TNFα causes increase in endothelial permeability as measured by the increase in FITC-dextran fluorescence intensity in all tested lines, data presented as means ±SEM, * p < 0.05, ** p < 0.01; (B) Increased permeability after stimulation with TNFα in hiPSC-ECs with mutation in HNF1A in comparison to the non-mutated cells, data is presented as percentage from the respective controls (cells cultured in complete medium without cytokine stimulation), where dots are means ± SEM, * p < 0.05; (C) Increased permeability after stimulation with TNFα in hiPSC-ECs with monoallelic or biallelic mutation in HNF1A in comparison to the non-mutated cells, data are presented as percentage from the respective controls (cells cultured in complete medium without cytokine stimulation), where dots are means ± SEM, * p < 0.05; ** p < 0.01.