Skip to main content
. 2019 Oct 24;9(11):859. doi: 10.3390/ani9110859

Figure 5.

Figure 5

ACVR1B knockdown promotes myogenic differentiation, but inhibits proliferation. (A) The transcript and protein levels of ACVR1B in PDGFRα- progenitor cells transfected with si-ACVR1B or si-NC. (B) The transcript and protein levels of MHC and MyoG on D5 of differentiation in bovine PDGFRα- progenitor cells transfected with si-ACVR1B or si-NC. (C) MHC-positive cells on D5 of myogenic differentiation in PDGFRα- progenitor cells transfected with si-ACVR1B and si-NC, as visualized by immunofluorescence. (D) EdU staining assay of PDGFRα- progenitor cells transfected with si-ACVR1B or si-NC, and the determination of EdU-positive cells. (E) Relative transcript levels of cell cycle-related genes in PDGFRα- progenitor cells transfected with si-ACVR1B or si-NC. Scale bars = 100 µm. All data are represented as mean ± standard deviation (SD), based on at least three independent experiments for each treatment. * p < 0.05 and ** p < 0.01.