Figure 5.

Gross locomotor ability in the Rotarod assay during pre-clinical EAE. Gait parameters were assessed in Control^Rotarod (n = 10) and EAE^Rotarod (n = 8) mice. Both cohorts were tested for their ability to run on a rotating cylinder that accelerated its speed with time (4–40 rpm in 300 s). Latencies to fall from the accelerating cylinder (i.e., latency), the maximum speed mice were able to run, and the walking distance on the rotating cylinder are presented as mean ± SEM. The D’Agostino and Pearson test was applied to test for normal distribution of the data. p-values for the effect of EAE treatment were calculated using t-test or Mann-Whitney test according to data distribution. ns = not significant.