Figure 3.

Conditioned medium of ADSC derived beige cells contains pro-angiogenic factors. Conditioned media were collected from confluent ADSCs and ADSC derived beige cells (Diff. ADSCs) cultured in basal medium for 48 h. (a) HUVEC cells (40,000 cells/cm²) were plated on reduced growth factor Cultrex and stimulated with conditioned medium obtained from ADSCs or ADSC derived beige cells. After 5 h, endothelial cell morphogenesis in terms of the formation of closed structures was examined using ImageJ software. Data are the mean ± SEM of three measurements per sample. * p < 0.05, Student’s t-test. (b) The Proteome Profiler Human Angiogenesis Antibody Array was used to detect angiogenesis related proteins in the conditioned medium of ADSCs and ADSC derived beige cells. Densitometry analysis of positive spot signals was normalized to positive and negative antibody array controls. Data are the mean of duplicate spots and expressed by color code (n = 2). Negative spots in both ADSCs and ADSC derived beige cell conditioned medium were not included in the analysis.