Figure A1.
(a) Commercial low, high molecular weight HA was electrophoretically analyzed on agarose gel and stained with Stains-All solution. p.a. means pre-autoclaved. Three different standard molecules (2 × 103, 1.1 × 103, 300 kDa) were used as markers of molecular weight (MK). (b) HUVEC spheroids embedded in collagen gel were stimulated with commercial HA. The formation of radially growing sprouts was evaluated after 24 h of incubation. Data are the mean ± SEM of three independent experiments (** p < 0.001, one-way ANOVA followed by Bonferroni’s test versus the control).