Skip to main content
. 2019 Nov 2;8(11):1847. doi: 10.3390/jcm8111847

Figure 6.

Figure 6

ODZ10117 induces apoptotic cell death and decreases migration and invasion of breast cancer. (A) Cells were incubated for 24 h with various concentrations of ODZ10117 and cell viability was determined. (B–E) MDA-MB-231 cells were incubated for 24 h with either vehicle (0.1% DMSO) alone or ODZ10117 (40 μM), and then performed FACS (B), immunoblot (C,D), and qPCR (E) analyses. 2D graphs indicate PI- (upper) or Annexin V-positive (bottom) cells. (F–I) MDA-MB-231 cells were incubated for 24 h with either vehicle (0.1% DMSO) alone or ODZ10117 (40 μM), and then performed wound healing (F) and Matrigel invasion (G) assays or immunoblot (H) and qPCR (I) analyses. Magnification, 200×. GAPDH served as a loading control. Data are represented as mean ± SEM of three independent experiments. * p < 0.05 and ** p < 0.005 compared to the vehicle-treated group.