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. 2019 Nov 15;8(11):1995. doi: 10.3390/jcm8111995

Figure 4.

Figure 4

Effect of acidification on the level of exosomal and non-exosomal proteins in ELV preparation. (A) Levels of non-exosomal proteins (GM130, calnexin, and ApoA-1) were scanty by treatment of proteinase K, while the acidification slightly increased the expression of calnexin and ApoA-1, but not GM130. (B,C) Among the exosomal markers (TSG101, CD63, Alix, and annexin 5), acidification increased the level of TSG101, Alix, and annexin 5, as compared with non-acidified, but proteinase-treated ELVs preparation, while the level of CD63 was comparable. Representative bands were shown (n = 6). * p < 0.05 vs. ELV with proteinase K 0.5 mg/mL without acidification; # <0.05 vs. ELV with proteinase 1.0 mg/mL without acidification, by Mann–Whitney U test.