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. 2019 Nov 15;8(11):1995. doi: 10.3390/jcm8111995

Table 1.

Quantitative PCR condition for amplification of the specific miRNAs.

miRNA Target Sequences Number of Cycles PCR Condition
miR-30c-5p 5′-UGUAAACAUCCUACACUCUCAGC-3′ 40 PCR initial heat activation; 95 °C, 2 min.
Denaturation; 95 °C, 10 sec.
Combined anneling/extention; 56 °C, 1 min.
miR-126-3p 5′-UCGUACCGUGAGUAAUAAUGCG-3′ 40
miR-192-5p 5′-CUGACCUAUGAAUUGACAGCC-3′ 40