Table 1.
miRNA | Target Sequences | Number of Cycles | PCR Condition |
---|---|---|---|
miR-30c-5p | 5′-UGUAAACAUCCUACACUCUCAGC-3′ | 40 | PCR initial heat activation; 95 °C, 2 min. Denaturation; 95 °C, 10 sec. Combined anneling/extention; 56 °C, 1 min. |
miR-126-3p | 5′-UCGUACCGUGAGUAAUAAUGCG-3′ | 40 | |
miR-192-5p | 5′-CUGACCUAUGAAUUGACAGCC-3′ | 40 |