Table 2.
Comparison of protein concentration and its variability according to the exosome-like extracellular vesicle (ELV) preparing methods. QG, miRCURY Exosome Serum/Plasma Kit; SBI, ExoQuick Exosome Isolation Kit for Serum and Plasma; LT, Invitrogen™ Total Exosome Isolation Kit.
| Kit | SBI (n = 6) | LT (n = 6) | QG (n = 6) | QG (n = 4) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Acidification | - | - | - | - | - | - | - | - | - | + | + |
| Proteinase K (mg/mL) | 0 | 0.5 | 1 | 0 | 0.5 | 1 | 0 | 0.5 | 1 | 0.5 | 1 |
| Protein concentration (mean, mg/mL) | 9.7 | 8.8 | 7.8 | 5.8 | 1.8 | 0.9 | 16.1 | 13.0 | 12.1 | 21.7 | 20.3 |
| %CV | 8.9 | 6.0 | 8.3 | 15.9 | 26.5 | 48.0 | 5.1 | 7.4 | 7.8 | 2.4 | 2.6 |