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. 2019 Oct 25;8(11):1319. doi: 10.3390/cells8111319

Figure 2.

Figure 2

NO regulates the hepatic insulin sensitivity in vivo. (A–C) Impact of modulating in vivo the NO production on hepatic insulin signaling. C57Bl6/JOlash male mice (n = 5 mice per condition) received ip injection of BH4 (12.5 mg/kg), l-Name (25 mg/kg), and a combination of the two, twice the day before and 2 h before the insulin test (0.75 U/kg). (A) Representative Western blots and quantitative analysis of insulin-stimulated (B) protein kinase B (Akt) and (C) glycogen synthase kinase 3 beta (GSK3β). (D) Impact of modulating in vivo the NO production on hepatic glucose production. C57Bl6/JOlash male mice (n = 8 mice per condition) received ip injection of BH4 (12.5 mg/kg), l-Name (25 mg/kg), and a combination of the two, twice the day before and 2 h before the pyruvate test (2 g/kg). £ and #, p < 0.05 vs. control. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. appropriate control glycaemia, respectively.