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. 2019 Nov 18;8(11):1460. doi: 10.3390/cells8111460

Figure 1.

Figure 1

Carbamazepine increases adipocyte differentiation in 3T3-L1 cells. (A) Chemical structure of carbamazepine (CBZ) [22]. (B) 3T3-L1 cells were treated with vehicle or carbamazepine at the indicated concentration. At day 7, differentiated cells were stained with Oil Red O. (Top) Photomicrographs of entire wells; (Bottom) Photomicrographs of stained cells (scale bar 100 μm). (C) Lipid accumulation in cells treated with different concentrations of carbamazepine. Absorbance of wells was measured by spectrophotometry and normalized with DNA content (n = 5 per group). (D) Cells were treated with vehicle or 40 μM carbamazepine. RNA samples at the indicated time points were prepared for RT-PCR. (E) Cells were differentiated until the indicated time point and levels of protein expression were analyzed by western blot. (F) Viability of 3T3-L1 cells treated carbamazepine for 24 h was analyzed by MTT assay (n = 5 per group). N.S. = not significant. Values are mean ± SEM. * P < 0.05.