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HIF-2α protein stability. (A) Hypoxic U87-GSCs were treated with 20 μg/mL CHX in absence or presence of 1 U/mL ADA for 0–4 h. Protein levels were analyzed by western blot and then plotted. (B) Same as in A, but ADA treatment was between 0–30 min. (C) Hypoxic U87-GSCs were treated with 10 μM MG132 and/or 1 U/mL ADA for 24 h and proteins were analyzed by western blot. n = 3.
