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. 2019 Oct 30;8(11):1353. doi: 10.3390/cells8111353

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of adenosine depletion on GSCs invasiveness. (A) Matrigel-coated transwell invasion assay of U87-GCSs in absence or presence of 1 U/mL ADA for 12 h. Cell invasion was plotted as fold change of the normoxic condition. (B) Same as in a but using PC-GSCs. (C) MMP-9 protein levels of U87-GSCs were analyzed by western blot and transcript levels of U87-GSCs and PC-GSCs were analyzed by RT-qPCR in the same treatmentconditions of A. (D) MMPs gelatinase activity was measured by zymography assay with GSCs extracellular medium. n = 3 * p < 0.05.