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. 2019 Oct 24;8(11):1771. doi: 10.3390/jcm8111771

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cellular activity of human chondrocytes following electrical stimulation with 1 kHz and either 100 mV or 1 V. Chondrocytes derived from non-degenerative (n = 4) or osteoarthritic (OA) cartilage (n = 6) were seeded on collagen scaffolds and stimulated over a period of seven days. Afterwards, metabolic activity was determined via water-soluble tetrazolium salt (WST-1) assay (a) and DNA content was analyzed by peqGOLD Tissue DNA Mini Kit (b). Data are presented as boxplots. Statistical analysis within a stimulation group was performed with Friedman test (^# p < 0.05). To compare two samples between OA and non-degenerative chondrocytes, Mann-Whitney-U-test was performed (* p < 0.05, ** p < 0.01).