Figure 1.

ZIKV mutant molecular clones. In (a), schematic representation of mutant viral clones BR15^E-MUT and MR766^E-MUT and their respective parental clones. In (b), histograms showing viral titres. Values represent means and standard errors of three independent experiments. In (c), examples of infectious plaques developed for BR15^E-MUT and MR766^E-MUT, and parental clones, after plaque-forming assay on Vero cells. In (d), Vero cells were infected with parental and mutant molecular clones (MR766 and BR15) at a MOI of 1. 24 h post-infection (hpi), cells were then lysed and subjected to an immunoblot, in non-reducing conditions. Anti-ZIKV EDIII immunoblot shows differences of electrophoretic mobility associated with residue mutations.