Figure 8.

TsES-pulsed DCs induced Treg cell differentiation and Th2 cytokine in naive CD4⁺ T cells in vivo. The splenocytes were isolated from naive BABL/c mice passively transferred with TsAES-, TsMES-, PBS-, and BSA-treated BMDCs. (A) The frequencies of CD4⁺CD25⁺Foxp3⁺ and CD4⁺CD25^-Foxp3⁺ T cells were measured by gated flow cytometry. (B) The percentages of CD4⁺CD25⁺Foxp3⁺ T cells and CD4⁺CD25^-Foxp3⁺ T cells are presented as the mean ± SEM of five individual mice per group. (C,D) Frequencies of CD4⁺ T cells producing IFN-γ⁺, (E,F) frequencies of CD4⁺ T cells producing IL-4^+, and (G,H) frequencies of CD4⁺ T cells producing IL-17A⁺ are shown. The data are presented as the mean ± SEM of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001 in a one-way analysis of variance followed by Bonferroni correction.