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. 2019 Nov 15;8(11):557. doi: 10.3390/antiox8110557

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Src/Stat3 signaling regulates the migratory ability of A431-III cells, which was reduced by the effectors. A wound-healing assay was conducted, and the cells were observed to migrate into the wound area. (A) A431-III cells were treated with dimethyl sulfoxide (DMSO), 10 and 20 μM of luteolin, 20 and 40 μM of quercetin, 1, 5 and 10 μM of Su6656, and 100, 200 and 400 μM of S3I-201. (B) The numbers of migrating cells treated with luteolin (a), quercetin (b), SU6656 (c) and S3I-201 (d) were measured from (A) and analyzed using ImageJ software (NIH, Bethesda, MA, USA). Statistical significance between groups were analyzed by a one-way ANOVA with Tukey’s test (* p < 0.05; ** p < 0.01; *** p < 0.001).